Coding

Part:BBa_K5366016

Designed by: Lin Li   Group: iGEM24_NJTECH-CHINA-A   (2024-09-27)


UxaE

Sequences encoding tagatose-4-epimerase activity derived from Thermotoga petrophila. UxaE was chosen as the template for the exploration of tagatose-4-epimerase, exhibiting a wild-type UxaE activity of 0.0006 U/mg. (In this context, enzyme activity is defined as the amount of enzyme that converts fructose to produce 1 µmol of tagatose per minute.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 501
    Illegal BglII site found at 1263
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 255
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None